Official Title
Genetic Polymorphisms and Their Association With Temporomandibular Disorders
Brief Title
Genetic Polymorphisms and Their Association With Temporomandibular Disorders
Protocol ID
NCT04694274
Lead Sponsor
Croatian Science Foundation
Brief Summary
Temporomandibular disorders (TMD) are the most common orofacial pain disorders of
non-dental origin with the prevalence of 6.1-10.2%, and incidence of 3.9%. Observable
pathology is mostly absent, and the etiology often remains unknown. Since some other
painful conditions of unknown origin (eg. fibromyalgia), also imply genetic factors, the
aim of the study is to investigate genetic predisposition in relation to the risk for TMD
onset. This will be achieved through analysis of polymorphisms in the selected genes in
TMD patients (DC/TMD) and matched control subjects. The possibility of involvement of
specific polymorphisms in modulation of therapy response will also be investigated. The
hypotheses: (I) the Single Nucleotide Polymorphism (SNPs) clustering will be dependent on
presence or absence of TMD (comparison of patients with control subjects), and will
possibly depend on source of pain, pain intensity, presence of bone changes,
psychological features and previous orthodontic therapy, and (II) SNPs will influence the
treatment response. Along with anamnestic and clinical examination and occlusal splint
therapy, genomic DNA will be analyzed from the buccal swabs. Isolated DNA will be used
for the determination of 19 polymorphisms of selected genes using Real-Time PCR method.
The analysis of salivary oxidative stress markers and opiorphin will be also performed,
as their relationship with TMD has been shown previously. This time, their concentration
will be associated with polymorphisms in the promoters of genes responsible for their
synthesis. The investigators expect to show that particular gene profile or group of SNPs
represent a risk factor for TMD development. Innovative approach of the concept of
determining the genetic predisposition for TMD has the potential for development of
commercial genetic test with potential for risk estimation in relation to TMD onset. This
could enable early interventions and active avoidance of environmental risk factors.
Study Period
-
Enrollment Count
60 participants
Eligibility Criteria
Inclusion Criteria:
- diagnosis of myofascial pain / arthralgia / painful disc displacement according to
Diagnostic Criteria for Temporomandibular Disorders (DC/TMD)
- average pain in the last 10 days >30 mm on a Visual Analogue Scale
- pain duration of at least 3 months
- good oral hygiene
- presence of own natural teeth
- absence of any form of chronic pain in the orofacial region or in other regions of
the body
Exclusion Criteria:
- other orofacial pain conditions including dental pain
- poor oral hygiene, gingivitis or periodontitis
- chronic medical conditions (diabetes, cardiovascular diseases, cancer, and
autoimmune diseases) - - -
- neurological and psychiatric disorders
- pregnancy
- causes of headache, unrelated to TMD, listed in the International Classification of
Headache Disorders
Filters
Temporomandibular Disorders
Orofacial Pain
UNKNOWN
CHILD
ADULT